A pair of primers were chemically synthesized based on the cdna sequence of hog cholera virus strain brescia and used to amplify the cdna fragments of envelope glycoprotein e2 gene of hclv which coding the major protective antigen by reverse transcription - polymerase chain reaction ( rt - pcr ) from total intact rna extracted from infected calf testicle cell culture by hclv 以猪瘟兔化弱毒犊牛睾丸细胞毒( hclv )中提取的细胞总rna为模板，以rt - pcr技术，扩增出了完整e2基因的cdna片段。经电泳、酶切分析，证实了所扩增片段为e2基因特异性片段。
